Indian Journal of Biochemistry & Biophysics

Indian Journal of Biochemistry & Biophysics

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The solvent system used was pyridine - aniline-water (9:1:4)] HPLC profile of fraction V is given in Fig. 4. This profile ... quots of 50 fx\ (approx. 400 fig per run) were loaded onto Aquapore RP-300 column, attached to a Shimadzu HPLC (C- R6A) with a variable wavelength detector. ... the gradient used. The absorbance of the eluate was monitored at 225 nm and the peak fractions were collected manually.

Title:Indian Journal of Biochemistry & Biophysics
Publisher: - 1992

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